Chemically Modified Primers for Improved Multiplexed PCR

Improved quantitative PCR using nested primers.

Quantitative PCR can often be improved by conducting the amplification with nested primers. First, fewer nonspecific amplification products, which could otherwise interfere with quantitation, are produced. Often, nonspecific products can be eliminated. In these cases, relatively simple nonspecific detection techniques are suitable for quantitation. In addition, nested primer PCR provides intrin...

Improved PCR specificity with Hot Start PCR primers.

The polymerase chain reaction (PCR) is an indispensable DNA amplification technique that has been exploited in numerous areas, including molecular diagnostics. One major drawback of PCR is the competing amplification of undesired off-target products, which primarily occurs at ambient temperatures prior to PCR cycling (1). Hot Start PCR techniques aim to block the extension of primers in nonspec...

Hot Start PCR with heat-activatable primers: a novel approach for improved PCR performance

The polymerase chain reaction (PCR) is widely used for applications which require a high level of specificity and reliability, such as genetic testing, clinical diagnostics, blood screening, forensics and biodefense. Great improvements to PCR performance have been achieved by the use of Hot Start activation strategies that aim to prevent DNA polymerase extension until more stringent, higher tem...

Tuning PCR specificity by chemically modified primer probes.

4766 [*] Dr. A. Marx, Dipl. Chem. M. Strerath Kekulÿ Institut f ̧r Organische Chemie und Biochemie Universit‰t Bonn Gerhard Domagk Strasse 1, 53121 Bonn (Germany) Fax: (þ 49)228 73 5388 E mail: a.marx@uni bonn.de [**] This work was supported by the DFG, the Volkswagenstiftung, and the Fonds der Chemischen Industrie. We thank Prof. Dr. M. Famulok for his continuing support and Prof. Dr. A. Pingou...

Designing PCR Primers Painlessly†